Avidin binding of radiolabeled biotin derivatives.
نویسندگان
چکیده
منابع مشابه
Amplified detection of avidin-biotin binding using terahertz wave technology
A new affinity biosensor based on a novel terahertz (THz) wave technology, differential time domain terahertz spectroscopy (DTDS), has been used to monitor the binding between biotin and avidin molecules. Amplified detection of ligandreceptor binding on supported membranes composed of a biotin bilayer on quartz modified octadecanol surface is a useful model lipid membrane for studying avidinbio...
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We have studied the structural elements that affect ligand exchange between the two high affinity biotin-binding proteins, egg white avidin and its bacterial analogue, streptavidin. For this purpose, we have developed a simple assay based on the antipodal behavior of the two proteins toward hydrolysis of biotinyl p-nitrophenyl ester (BNP). The assay provided the experimental basis for these stu...
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Atomic force microscopy of single molecules, steered molecular dynamics and the theory of stochastic processes have established a new field that investigates mechanical functions of proteins, such as ligand–receptor binding/unbinding and elasticity of muscle proteins during stretching. The combination of these methods yields information on the energy landscape that controls mechanical function ...
متن کاملA Spectrophotometric Assay for Avidin and Biotin Based on Binding of Dyes by Avidin.
The binding of aniohic dyes by serum albumin has been studied by many workers (reviewed by Foster, 1960). The evidence suggests several cationic sites situated in hydrophobic regions of the molecule. For example, the bound dye shows spectral changes that are in accord with its location in a non-polar environment. Since it was suggested (Green, 1963b) that the biotin-binding sites of avidin were...
متن کاملChicken avidin-related proteins show altered biotin-binding and physico-chemical properties as compared with avidin.
Chicken avidin and bacterial streptavidin are proteins familiar from their use in various (strept)avidin-biotin technological applications. Avidin binds the vitamin biotin with the highest affinity known for non-covalent interactions found in nature. The gene encoding avidin (AVD) has homologues in chicken, named avidin-related genes (AVRs). In the present study we used the AVR genes to produce...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1988
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)57380-x